Non-Conventional Method

Artificial induction of agarwood formation is the use of chemical, insect and pathogen-inducing techniques is increasingly common in agarwood induction. Chemical inducers normally comprise of phytohormones, salts, minerals and biological-derived substances. Various chemical induction approaches are developed, including cultivated agarwood kit (CA-kit), the whole-tree agarwood inducing technique (Agar-Wit) and biologically agarwood-inducing technique (Agar-bit). CA-kit is a combined method based on physical wounding and chemical induction, where the inducing agent is applied into the Aquilaria tree via an aeration device inserted into the wound. Agar-Wit is a transpiration-assisted chemical treatment to form an overall wound in the tree, where the preloaded inducer in a transfusion set is distributed via plant transpiration.

Similarly, Agar-bit method adopts the idea of distributing the inducing reagent by plant transpiration, except that the reagents are injected directly into the stems of the tree. Chemical inducers are suitable for mass production of agarwood with easier quality control than biological inoculum. However, in spite of the fast results and high yields, the application of chemical inducers still poses skepticism of toxicity on both human and environment. All of these induction techniques in any case mimic the natural processes of agarwood formation, which have their own strengths and weaknesses. On the other hand, in vitro culture of various parts of Aquilaria spp. and Gyrinops spp. are studied at various tissue culture laboratories. The tissue culture techniques identified the key regulator genes of Aquilaria spp. and Gyrinops spp. involved in the agarwood production.